Baicalein induces the apoptosis of gastric cancer MGC-803 cells
10.3781/j.issn.1000-7431.2017.11.369
- Author:
Peilin SUN
1
Author Information
1. Cancer Research Center, Yanbian University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Baicalein;
Cell proliferation;
Stomach neoplasms
- From:
Tumor
2017;37(10):1041-1046
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of baicalein (BAI) on the apoptosis of gastric cancer MGC-803 cells. Methods: After MGC-803 cells were treated with different concentrations of BAI, the proliferation and cell apoptosis of MGC-803 cells were detected by MTT and FCM assays, respectively. The cell apoptosis bodies formation of MGC-803 cells after treatment with different concentrations of BAI was observed by Hoechst 33342 staining, and the expression levels of the apoptosis-related proteins of MGC-803 cells were detected by Western blotting. Results: The 5, 10, 15, 25 and 50 mol/L BAI could inhibite the proliferation of gastric cancer MGC-803 cells (all P < 0.05). The apoptotic rate of gastric cancer MGC-803 cells after treatment with 10, 15 and 25 mol/L of BAI was increased (all P < 0.05). The number of apoptotic bodies in gastric cancer MGC-803 cells after treatment with 10, 15 and 25 mol/L of BAI was increased by Hoechst 33342 staining (all P < 0.05). The expression levels of cleaved-caspase-3 and cleaved-poly ADP-ribose polymerase (PARP) in MGC-803 cells were up-regulated after treatment with BAI, and the expression level of Bcl-2 was down-regulated (all P < 0.01). Conclusion: BAI can suppress the proliferation of gastric cancer MGC-803 cells, and induce the cell apoptosis. The mechanism may be related to the regulation of the expressions of the apoptosis-related proteins including caspase-3, PARP and Bcl-2 by BAI.
