microRNA-378 promotes proliferation and migration of renal carcinoma cells and its mechanism
10.3781/j.issn.1000-7431.2018.11.111
- Author:
Na AN
1
Author Information
1. Renji-Med X Clinical Stem Cell Research Center, Renji Hospital, Shanghai Jiao Tong University, School of Medicine
- Publication Type:Journal Article
- Keywords:
Carcinoma;
Cell migration assays;
Cell proliferation;
Gene expression regulation;
Large tumor suppressor 2;
MicroRNAs;
MiR-378;
Renal cell
- From:
Tumor
2018;38(7):643-651
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of microRNA-378 (miR-378) on proliferation and migration of renal cancer cell lines in vitro, and to explore the possible mechanism. Methods: The expression of miR-378 in renal cell carcinoma and normal renal epithelial tissues was analyzed using the datasets from The Cancer Genome Atlas (TCGA). The expression of miR-378 in human renal carcinoma cell lines 786-O, ACHN, 769-P and Caki-1 as well as human renal proximal tubular epithelial cell line HK2 was detected by real-time fluorescent quantitative PCR (RFQ-PCR). MiR-378 mimic and inhibitor were synthesized and then transfected into renal carcinoma 786-O and Caki-1 cells, respectively. The proliferation and migration of 786-O and Caki-1 cells were detected by CCK-8 assay, Transwell chamber assay and wound healing assay, respectively. The potential target gene of miR-378 was screened by bioinformatics, and confirmed by RFQ-PCR and Western blotting in renal cancer cells, respectively. Results: The expression level of miR-378 in renal cancer tissues and cell lines was significantly up-regulated as compared with that in normal renal epithelial tissues or normal renal epithelial HK2 cells (all P < 0.001). After transfection with miR-378 mimic, the proliferation (P < 0.01) and migration (P < 0.001) of 786-O cells were remarkably increased. In contrast, after transfection with miR-378 inhibitors, the proliferation (P < 0.01) and migration (P < 0.001) of Caki-1 cells were remarkably suppressed. By bioinformatics assay, the large tumor suppressor 2 (LATS2) was identified as a target gene of miR-378 in renal cancer. The mRNA and protein expression of LATS2 were negatively related with the expression of miR-378 in renal cancer 786-O and Caki-1 cells (all P < 0.01). Conclusion: Overexpression of miR-378 may promote the proliferation and migration of renal cancer cells by negative targeting LATS2 gene.
