CAFs conditioned medium up-regulates the expression of survivin gene in human intrahepatic cholangiocarcinoma HCCC-9810 cells
10.3781/j.issn.1000-7431.2019.11.161
- Author:
Yonghong MA
1
Author Information
1. Department of Hygiene, School of Public Health, Xi’an Medical University
- Publication Type:Journal Article
- Keywords:
Cancer-associated fibroblasts;
Gene expression;
Liver neoplasms;
Survivin
- From:
Tumor
2019;39(7):542-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of carcinoma-associated fibroblasts (CAFs) conditioned medium on the expression of survivin gene in human intrahepatic cholangiocarcinoma HCCC-9810 cells. Methods: The effect of CAFs conditioned medium on the proliferation of HCCC-9810 cells was detected by MTT assay. The effect of CAFs conditioned medium on the cell cycle distribution of HCCC-9810 cells was detected by FCM. The effects of CAFs conditioned medium on the expression levels of survivin mRNA and protein were detected by realtime fluorescent quantitative PCR and Western blotting, respectively. Results: CAFs conditioned medium significantly promoted the proliferation of HCCC-9810 cells (P < 0.05). The percentage of HCCC-9810 cells at G0/G1 phase in CAFs conditioned medium group was lower than that in the control group (cultured with RPMI 1640 medium) (P < 0.05), while the percentage of HCCC-9810 cells at S phase was opposite (P < 0.05). The percentage of HCCC-9810 cells at G2/M phase had no difference between CAFs conditioned medium group and the control group (P > 0.05). The expression levels of survivin mRNA and protein in HCCC-9810 cells were up-regulated by CAFs conditioned medium (both P < 0.05). Conclusion: CAFs conditioned medium can promote the proliferation and cell cycle progression of HCCC-9810 cells. This effect is related to the up-regulaion of survivin mRNA and protein expressions.
