Expression of microRNA-1299 in esophageal carcinoma and its effects on migration and invasion of esophageal cancer cells
10.3781/j.issn.1000-7431.2019.11.341
- Author:
Xuemei MENG
1
Author Information
1. Dosing Center of Pharmacy, Fourth Hospital of Hebei Medical University
- Publication Type:Journal Article
- Keywords:
Cell movement;
Cell proliferation;
Esophageal neoplasms;
MicroRNA-1299
- From:
Tumor
2019;39(8):623-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of microRNA-12 99 (miR-1299) in esophageal carcinoma and its effects on the migration and invasion of esophageal cancer TE1 cells. Methods: The pathological tissue specimens and their matched paracancerous tissues of 58 patients with esophageal cancer were selected in the Fourth Hospital of Hebei Medical University from June 2017 to June 2018. The expression level of miR-1299 in cancer and corresponding adjacent tissues was detected by real-time fluorescent quantitative PCR. The relationship between miR-1299 expression and clinicopathological features was analyzed. After miR-1299-mimics were transfected into esophageal cancer TE1 cells for the overexpression of miR-1299, the proliferation of TE1 cells was detected by CCK-8 assay, and the migration and invasion abilities were detected by Transwell migration and invasion assays, respectively. Then the expressions of matrix metalloproteinase 11 (MMP11) and MMP16 mRNAs and proteins in miR-1299 over-expressed TE1 cells were detected by realtime fluorescent quantitative PCR and Western blotting, respectively. Results: The expression of miR-1299 in esophageal cancer tissues was significantly lower than that in the adjacent tissues (P< 0.001). The expression level of miR-1299 was associated with the clinical stage of esophageal cancer patients (P < 0.05). After the transfection of miR-1299-mimics into TE1 cells, the expression level of miR-1299 was significantly increased (P< 0.01). After the over-expression of miR-1299 in TE1 cells, the proliferation of TE1 cells did not change significantly (P > 0.05), while the migration (P < 0.001) and invasion (P < 0.01) abilities decreased significantly, and the expressions of MMP11 and MMP16 mRNAs and proteins were obviously decreased (all P< 0.01). Conclusion: The expression of miR-1299 is down-regulated in esophageal cancer tissues and may inhibit the migration and invasion of esophageal cancer cells.
