Expression and mechanism of long non-coding RNA DLGAP1-AS2 in gastric cancer
10.3781/j.issn.1000-7431.2020.11.1007
- Author:
Jiawei LU
1
Author Information
1. Department of General Surgery, Affiliated People’s Hospital of Jiangsu University
- Publication Type:Journal Article
- Keywords:
DLGAP1-AS2;
Epithelial-mesenchymal transition;
Long non-coding;
RNA;
Stomach neoplasms
- From:
Tumor
2020;40(3):153-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of long non-coding RNA (lncRNA) DLGAP1-AS2 in gastric cancer and its clinical significance. Methods: The data set of gastric adenocarcinoma was downloaded from The Cancer Genome Atlas (TCGA) database, and the difference of DLGAP1-AS2 expression between tumor tissues and normal tissues was analyzed. The tumor and adjacent tissues were collected from 85 gastric adenocarcinoma patients, and the plasma samples were collected from 25 gastric cancer patients and 25 healthy controls. Then the expression of DLGAP1-AS2 in tumor tissues and plasma samples was detected by real-time fluorescent quantitative PCR, and the correlation of the level of DLGAP1-AS2 with the clinicopathological features of gastric cancer patients was analyzed. Besides, after the transfection with DLGAP1-AS2 siRNA, the proliferation, migration and invasion of gastric cancer AGS cells were observed by viable cell counting method, colony formation assay and Transwell chamber assay, respectively. Moreover, the expressions of epithelial-mesenchymal transition (EMT)-related molecules after silencing DLGAP1-AS2 in AGS cells were detected by Western blotting. Results: The expression of DLGAP1-AS2 in gastric cancer tissues was higher than that in normal gastric epithelium according TCGA database (P < 0.001). DLGAP1-AS2 was significantly overexpressed in gastric cancer tissues as compared with the corresponding paracancerous tissues, which was correlated with the advanced TNM stage, lymph node metastasis and overall survival rate (all P < 0.05). While the level of DLGAP1-AS2 was also upregulated in the plasma of patients with gastric cancer as compared with the healthy (P < 0.05). Knockdown of DLGAP1-AS2 expression inhibited the proliferation, colony formation, migration and invasion of gastric cancer AGS cells (all P < 0.05). In addition, the expression levels of matrix metalloproteinase 2 (MMP2), Slug, N-cadherin (N-cad) and Twist were significantly down-regulated in AGS cells transfected with DLGAP1-AS2 siRNA (all P < 0.05). Conclusion: The expression of DLGAP1-AS2 is significantly increased in tumor tissues and plasma of gastric cancer patients. DLGAP1-AS2 knockdown can reduce the proliferation, migration and invasion of gastric cancer cells, and its action mechanism may be related to the regulation of EMT-related genes expression.
