Effects of 1,25(Oh)2d3 on microrna-130b and transforming growth factor beta 1 in renal tissue of rat models of diabetic nephropathy
10.3969/j.issn.2095-4344.1907
- Author:
Yuetong LIU
1
Author Information
1. Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University
- Publication Type:Journal Article
- Keywords:
1,25(OH)2D3;
Diabetic nephropathy;
Kidney fibrosis;
MicroRNA-130b;
SPF Sprague-Dawley rats;
Streptozotocin;
Transforming growth factor pi
- From:
Chinese Journal of Tissue Engineering Research
2020;24(2):248-253
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: 1,25(OH)2D3 plays an important regulatory role in the development of diabetic nephropathy. OBJECTIVE: To explore the effect of 1,25(OH)2D3 on the expression of microRNA-130b and transforming growth factor pi in kidney of diabetic nephropathy rats. METHODS: The study was approved by the Laboratory Animal Ethical Committee of Laboratory Animal Center of Xinjiang Medical University. Twenty-five clean Sprague-Dawley rats were randomly divided into normal control group, diabetic nephropathy+1,25(OH)2D3 group and diabetic nephropathy+peanut oil group. The latter two groups were given calcitriol (1,25(OH)2D3, active vitamin D3, 0.03 jg/kg^d) treatment and peanut oil control treatment, respectively. After 37 days, the samples were collected and the expression of transforming growth factor pi in rat kidney tissue was detected by RT-PCR, western blot assay and immunohistochemical staining. The expression level of microRNA-130b in kidney was detected by RT-PCR. Morphological structure and degree of fibrosis of rat kidney were analyzed by hematoxylin-eosin staining and Masson staining. RESULTS AND CONCLUSION: (1) RT-PCR results showed that the expression levels of microRNA-130b in the diabetic nephropathy+ 1,25(OH)2D3 group and diabetic nephropathy+peanut oil group were significantly lower than those in the normal control group (P < 0.01). The level of microRNA-130b in the diabetic nephropathy+1,25(OH)2D3 was significantly higher than that in the diabetic nephropathy+peanut oil group (P < 0.01). (2) RT-PCR, western blot assay and immunohistochemical staining and pathological findings showed that the expression level of transforming growth factor pi mRNA protein and renal tissue structure disorder and degree of fibrosis in the diabetic nephropathy+1,25(OH)2D3 group and diabetic nephropathy+peanut oil group were significantly higher than those in the normal control group (P < 0.01). The level of transforming growth factor mRNA protein and renal tissue structure disorder and degree of fibrosis in the diabetic nephropathy+1,25(OH)2D3 group were significantly lower than those in the diabetic nephropathy+peanut oil group (P < 0.01). (3) These results suggest that the expression level of microRNA-130b is decreased and transforming growth factor pi mRNA and protein levels are increased in renal tissue of diabetic nephropathy rats and the renal tissue structure is disordered and the degree of fibrosis is severe. 1,25(OH)2D3 can up-regulate the expression of microRMA-130b and also down-regulate the expression of transforming growth factor pi in the kidney of diabetic nephropathy rats, thus improving the degree of renal tissue structure disorder and fibrosis.
