D3 and D5 Dopamine Receptor mRNA Expression in Peripheral Blood Mononuclear Cells from Patients with Parkinson's Disease.
- Author:
Jin Soo KIM
1
;
Young Ho SOHN
;
Yong Tae KWAK
;
Chul Hee CHOI
;
Sun Ah CHOI
;
Dong Chul PARK
;
In Hong CHOI
Author Information
1. Department of Neurology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Parkinson's disease;
Peripheral blood mononuclear cell;
Dopamine receptor;
messenger RNA;
PCR
- MeSH:
Actins;
Dopamine*;
Humans;
Parkinson Disease*;
Polymerase Chain Reaction;
Receptors, Dopamine*;
Reverse Transcription;
RNA, Messenger*
- From:Journal of the Korean Neurological Association
1999;17(3):359-364
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Among 5 subfamilies of dopamine receptors (DAR), D3 and D5 DAR are expressed on peripheral blood mononuclear cells (PBMC). Recently, those DARs have been reported to change in Parkinson's disease (PD). METHODS: We measured the DAR mRNA expression in PBMC from 15 PD patients who had never taken antiparkinson medication, and 16 age-matched healthy people by reverse transcription and quantitative competitive polymerase chain reaction. The beta-actin mRNA expression was also measured to evaluate the relative expression of DAR mRNA. RESULTS: The D3 and D5 DAR mRNA expression was not different between patients and controls. In patients, no significant cor-relation was found between DAR mRNA expression in PBMC and clinical variables such as severity and duration of symptoms, and patients' age. CONCLUSIONS: We confirmed the presence of D3 and D5 DAR in PBMC. However, their mRNA expressions were not influenced by the disease process of PD.
