Nerve growth factor interferes with proliferation and alpha-actin expression of skeletal muscle satellite cells in rats
10.3969/j.issn.2095-4344.3513
- Author:
Nan YAN
1
Author Information
1. Shenyang Medical College
- Publication Type:Journal Article
- Keywords:
Actin;
Bone;
Cell culture;
Cell proliferation;
Factor;
Nerve growth factor;
Satellite cell;
Skeletal muscle
- From:
Chinese Journal of Tissue Engineering Research
2020;25(13):2030-2035
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Clinical studies have confirmed that the injection of nerve growth factor into the nerve damage site can improve the motor function of skeletal muscle. OBJECTIVE: To observe the effect of nerve growth factor on the proliferation of primary skeletal muscle satellite cells in rats. METHODS: The original generation of rat skeletal muscle satellite cells were cultivated by the tissue block method, and the third generation of skeletal muscle satellite cells were divided into three groups. The control group was added with culture medium; the low concentration group and the high concentration group were added with culture medium containing 10, 20 U/mL nerve growth factors, respectively. At 2, 4 and 6 days after culture, the cell morphology was observed by inverted phase contrast microscope and hematoxylin-eosin staining, and the expression of α-actin was observed by immunohistochemistry. At 1, 2, 3, and 4 days after culture, CCK-8 assay was used to detect the cell proliferation activity. RESULTS AND CONCLUSION: (1) Inverted phase contrast microscope displayed that with the increase of culture time, the number of skeletal muscle satellite cells in each group increased gradually, and the cell morphology gradually changed from round to fusiform, spindle or polygonal, and gradually fused and arranged along the same direction, and the number of cells in high concentration group was more than that in low concentration group and control group (P < 0.05). (2) Hematoxylin-eosin staining showed that the outline of satellite cells in skeletal muscle of each group was clear and densely distributed, and the nucleus was large and deeply stained; there was no significant difference among groups. (3) Immunohistochemical results showed that the expression of α-actin in skeletal muscle in each group was positive, but there was no statistical difference in the optical density of α-actin positive particles in each group (P > 0.05). (4) The CCK-8 assay results showed that cell viability of low and high concentration groups was higher than that of control group (P=0.000). (5) Above results confirm that high and low concentrations of nerve growth factors could promote the proliferation of skeletal muscle satellite cells, but had no effect on the expression of α-actin in skeletal muscle satellite cells.
