Cell labeling and tracking imaging in vivo: Newest advance in animals and humans
10.3969/j.issn.2095-4344.2077
- Author:
Meng Xin XU
1
Author Information
1. College of Chemistry and Molecular Engineering, Peking University
- Publication Type:Journal Article
- Keywords:
Cell imaging;
Cell labeling;
Fluorescence imaging;
MRI;
PET;
SPECT
- From:
Chinese Journal of Tissue Engineering Research
2020;24(19):3108-3116
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Various cell therapy products have been approved by clinical trials worldwide, and cell therapies such as stem cell therapy and adoptive immunotherapy have attracted much attention. Real-time observation and imaging in vivo can visualize the distribution of cells, track cell movement, monitor cell viability, and observe the cell migration and growth. Many imaging technologies can visualize cells in vivo, such as ultrasound, optics, MRI and nuclear imaging, and these methods need to correspond to different labeling and detection strategies. Each strategy has its own advantages and disadvantages. OBJECTIVE: To review the principle and development of different tracking methods, and their application in animals and humans. METHODS: PubMed, Google Scholar, Web of Science and CNKI databases were searched with the keywords of “cell tracking, in vivo cell tracking, PET imaging, MRI, optical imaging.” The articles published in the past 5-10 years were preferred. The contents of the articles mainly describe the principle of different tracking methods, and their application in animal models and patients. RESULTS AND CONCLUSION: In the past 20 years, cell tracking has developed into a multifarious discipline, not only establishing a variety of robust methods in animal models, but also proving the feasibility of clinical transformation in some human studies. The development of the non-invasive detection methods, such as PET and MRI, and new contrast agents provides strong support for the application of cell therapy in clinical and scientific researches.
