Effect of continuous loading pressure on apoptosis and Wnt/beta-catenin signaling pathway in nucleus pulposus cells
10.3969/j.issn.2095-4344.2762
- Author:
Xun Lu YIN
1
Author Information
1. Wangjing Hospital of China Academy of Chinese Medical Sciences
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Continuous loading pressure;
Nucleus pulposus cells;
Spinal motion segment;
Wnt/β-catenin signaling pathway
- From:
Chinese Journal of Tissue Engineering Research
2020;24(26):4125-4128
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Previous studies have found that continuous loading pressure can induce intervertebral disc degeneration by reducing the number of nucleus pulposus cells and reducing the expression of extracellular matrix in the nucleus pulposus. However, its mechanism of action is unclear. OBJECTIVE: To study the effect of continuous loading pressure on apoptosis and Wnt/β-catenin signaling pathway in nucleus pulposus cells. METHODS: The in vitro models of rabbit spinal motion segment under loading pressure were established and divided into control group (no treatment), pressure group (3 kg continuous loading pressure) and observation group (3 kg continuous loading pressure + 20 μmol/L SB216763). After 3 days of intervention, the pathological changes of nucleus pulposus cells were detected by hematoxylin-eosin staining, and the expression of caspase-3 and GSK-3β protein in nucleus pulposus tissue was detected by western blot assay.
