Tendon-bone healing in anterior cruciate ligament reconstruction with and without remnant preservation
10.3969/j.issn.2095-4344.2373
- Author:
Zi Yue MA
1
Author Information
1. First Department of Joint Surgery, Sixth Affiliated Hospital of Xinjiang Medical University
- Publication Type:Journal Article
- Keywords:
anterior cruciate ligament;
Bone tunnel;
Experiment;
Factor;
Microvessel density;
Remnant preservation;
Tendon-bone healing
- From:
Chinese Journal of Tissue Engineering Research
2021;25(4):582-587
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Whether remnant preservation can improve tendon-bone healing remains a controversy. Experiments were designed to evaluate whether remnant preservation has the biological advantage of promoting tendon-bone healing by histology and imaging. OBJECTIVE: To investigate the effect of preservation of ligament stump on tendon-bone healing in anterior cruciate ligament reconstruction. METHODS: Forty New Zealand rabbits were randomly divided into two groups: anterior cruciate ligament reconstruction group without remnant preservation (group A) and anterior cruciate ligament reconstruction group with remnant preservation (group B), with 20 rabbits in each group. Achilles tendon was selected as the graft, and the bilateral anterior cruciate ligaments of all the rabbits were cut off. In group A, the anterior cruciate ligament stump was completely removed. In group B, the tibia stump was cut off from the femoral stop, and the tibia stump was retained. According to the position of the anterior cruciate ligament of the normal rabbits, the tibia and the femoral canal were selected for reconstruction. At 6 and 12 weeks after surgery, the expression of vascular endothelial growth factor and hypoxia inducible factor-1α was detected by immunohistochemistry. Graft microvessel density was detected by CD34 immunohistochemical staining. The signal intensity of tendon and the width of bone tunnel were observed by MRI and CT. RESULTS AND CONCLUSION: (1) The percentage of hypoxia inducible factor-1α and vascular endothelial growth factor positive cells in group B was significantly higher than that in group A at 6 weeks after operation (P < 0.05). The microvessel density in group B was significantly higher than that in group A (P < 0.05). At 12 weeks after operation, the percentage of hypoxia inducible factor-1α and vascular endothelial growth factor positive cells in the two groups decreased 12 weeks after operation compared with that in the same 6-week group (P < 0.05). The microvessel density in the two groups increased 12 weeks after operation compared with that in the same 6-week group (P < 0.05), and there was no significant difference between the two groups (P > 0.05). (2) At 6 weeks after operation, the expansion of bone tunnel in group B was significantly lower than that in group A, and the signal intensity of tendon graft was lower in group B than that in group A (P < 0.05). After 12 weeks, the expansion degree of bone tunnel and the signal intensity of tendon were decreased in both groups (in the same group P < 0.05, between the two groups P > 0.05). (3) In this experiment, in the early stage of ligament reconstruction, the anterior cruciate ligament reconstruction with remnant preservation is superior to the anterior cruciate ligament reconstruction without remnant preservation in terms of graft revascularization and reduction of bone tunnel expansion, showing some biological advantages.
