The Therapeutic Effects of Combination Therapy with Curcumin and Alendronate on Spine Fusion Surgery in the Ovariectomized Rats.
10.14245/kjs.2017.14.2.35
- Author:
Dae Chul CHO
1
;
Kyoungsu RYU
;
Kyoung Tae KIM
;
Joo Kyung SUNG
Author Information
1. Department of Neurosurgery, Kyungpook National University School of Medicine, Daegu, Korea. dccho@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Bone fusion;
Curcumin;
Osteoporosis;
Ovariectomy;
Rat
- MeSH:
Alendronate*;
Animals;
Curcumin*;
Female;
Humans;
Osteoporosis;
Ovariectomy;
Palpation;
Rats*;
Rats, Sprague-Dawley;
Spine*;
Therapeutic Uses*
- From:Korean Journal of Spine
2017;14(2):35-40
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The purpose of this study was to evaluate the therapeutic effects of combination therapy with curcumin and alendronate on spine fusion surgery in ovariectomized rats. METHODS: Thirty-two female Sprague-Dawley rats (12 weeks old) underwent bilateral ovariectomy (OVX). Eight weeks after surgery, animals underwent intertransverse spine fusion at L4–5. The rats were randomly distributed amongst 4 groups; untreated OVX group, curcumin administered group, alendronate administered group, and the combination therapy group. At 8 weeks after fusion surgery, the animals were sacrificed and the fusion mass was assessed by manual palpation, radiographic scan, and micro-computed tomographic scan. In addition, mechanical strength was determined by a 3-point bending test. RESULTS: Based on the results of manual palpation testing and 3-dimensional micro-computed tomography scanning, solid bone fusion rate was 50% (4 of 8) in the OVX group, 75% (6 of 8) in the alendronate-only and curcumin-only group, and 87.5% (7 of 8) in the combination therapy group, respectively. The combination therapy group had a higher fusion rate compared with the other treatment groups, though not statistically significantly (p>0.05). And the combination therapy group had a significant increase in fusion volume at 8 weeks after spine fusion surgery compared with curcumin-only group (p=0.039). The 3-point bending test showed that combination therapy group had a significantly greater maximal load value compared to that of curcumin-only group (p=0.024). CONCLUSION: The present study demonstrated that additional treatment of curcumin and alendronate after spine fusion surgery in rat can promote higher fusion volume, and improve bone mechanical strength.
