UHPLC-MS-MS high throughput analysis-based screening and evaluation of PXR/CYP3A4-induced lipid-regulating quality marker in propolis
10.7501/j.issn.0253-2670.2020.03.016
- Author:
Zhao CHEN
1
Author Information
1. The Fifth College of Clinic Medicine, Guangzhou University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Caffeic acid phenethylester;
Chrysin;
Galangin;
Propolis;
PXR/CYP3A4;
Quality markers;
Quercetin
- From:
Chinese Traditional and Herbal Drugs
2020;51(3):662-668
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen and evaluate PXR/CYP3A4-induced lipid-regulating quality marker in propolis with precise and quantitative method. Methods: The LS174T cell was given certain amount of midazolam injection, along with different dosage of known components found in propolis, after incubation and extraction, the samples were determined for 1’-OH-midazolam, and each compound was evaluated to discover the PXR/CYP3A4 pathway regulatory activity according to the results; Then, compounds selected were used as indexes for UHPLC-MS-MS content determination, and their own values were regarded as a preliminary step of confirming PXR/CYP3A4-induced lipid-regulating quality markers of propolis. Results: In all components tested, chrysin, galangin, heterochlorogenic acid A, quercetin, and caffeic acid phenethylester significantly affected the 1’-OH-midazolam yield compared with blank and positive control, indicating their obvious influence on PXR/CYP3A4 expression; The UHPLC-MS-MS determination showed that except galangin, heterochlorogenic acid A, and quercetin, all the other compounds had adequate content in propolis to take effect. Conclusion: Chrysin, galangin, caffeic acid phenethylester, and quercetin were probably defined as PXR/CYP3A4-induced lipid-regulating quality marker in propolis, which inhibited the expression of such targets to down-regulate blood lipid level; Additionally, the method used for quality marker screening and evaluation in this study was fast, effective and quantitative, and capable of carrying out high throughput active component screening for PXR/CYP3A4 regulatory activities.
