Mechanism of ginsenoside CK inhibiting proliferation of human colon cancer SW480 cells
10.7501/j.issn.0253-2670.2020.06.025
- Author:
Xue MENG
1
Author Information
1. School of Pharmacy, Changchun University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cell apoptosis;
Cell proliferation;
Cytochrome C;
Ginsenoside compound K;
Human colon cancer SW480 cells;
Mitochondria
- From:
Chinese Traditional and Herbal Drugs
2020;51(6):1567-1574
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of ginsenoside CK on proliferation and apoptosis of human colon cancer cell line SW480, and further explore the mechanism. Methods: Cell viability was measured by CCK-8 assay. Cell cycle, apoptosis, reactive oxygen species (ROS) levels and changes in mitochondrial membrane potential were measured by flow cytometry. Hoechst staining further detected apoptosis. Western blotting was used to detect the release of cytochrome C and the expression of apoptosis-related proteins such as Bcl-2, Bax and cleaved Caspase-3. Results: Ginsenoside CK had a significant inhibitory effect on the proliferation of human colon cancer cell line SW480. Ginsenoside CK induced SW480 cells arrest in G0/G1 phase, promoted early apoptotic cells, significantly increased intracellular ROS levels and reduced the MMP level. Ginsenoside CK promoted the expression of Bax and cleaved-Caspase-3 and inhibited the expression of Bcl-2. In addition, ginsenoside CK released a large amount of cytochrome C in SW480 cells. Conclusion: Ginsenoside CK has a significant inhibitory effect on the proliferation of human colon cancer cell line SW480. The mechanism may be through the promotion of mitochondrial superoxide elevation, resulting in a significant increase in intracellular ROS levels and a significant decrease in MMP level, further leading to the release of cytochrome C, the up-regulated expression of Bax, the down-regulated expression of Bcl-2, and ultimately leading to apoptosis of cells.
