Simultaneous determination of six components in Mongolia medicine Gurigumu-13 Pills by QAMS
10.7501/j.issn.0253-2670.2020.06.022
- Author:
Guo-Dong WU
1
Author Information
1. Baotou Medical College
- Publication Type:Journal Article
- Keywords:
Costunolide;
Dehydrocostus lactone;
Ellagic acid;
Gallic acid;
Geniposide;
Gurigumu-13 Pill;
Hydroxysafflor yellow A;
Quantitative analysis of multi-components by single marker (QAMS)
- From:
Chinese Traditional and Herbal Drugs
2020;51(6):1542-1547
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method for the simultaneous determination of six components of gallic acid, hydroxysafflor yellow A, geniposide, ellagic acid, costunolide and dehydrocostus lactone in Gurigumu-13 Pill, which is proved to be a scientific and feasible method in the quality analysis in Gurigumu-13 Pill. Methods: The relative factor (fs/i) of gallic acid, ellagic acid, hydroxysafflor yellow A, costunolide and dehydrocostus lactone were established by HPLC method with geniposide as internal standard, which were used to calculate the content of five constituents in the samples of Gurigumu-13 Pill. Meanwhile, external standard method (ESM) was used to calculate the content of six constituents. The difference between QAMS and ESM was analyzed to evaluate the accuracy of QAMS. Results: The fs/i of gallic acid, hydroxysafflor yellow A, ellagic acid, costunolide and dehydrocostus lactone were 0.481 0, 0.906 4, 0.170 9, 0.971 2 and 1.261 5, respectively. The content determination results of six batches of Gurigumu-13 Pill were calculated by the method of QAMS and ESM, with no significant difference in RSD < 2.0%. Conclusion: The fs/i established in the QAMS method with geniposide as the internal reference substance is accurate and simple. The QAMS method can be used for the multi-index quality evaluation of Gurigumu-13 Pill.
