Spatiotemporal regulation of fibroblast growth factor signal blocking for endoderm formation in Xenopus laevis.
10.3858/emm.2008.40.5.550
- Author:
Sang wook CHA
1
;
Jong Woo LEE
;
Yoo seok HWANG
;
Jeong Pil CHAE
;
Kwon Moo PARK
;
Hee Jung CHO
;
Dong Sun KIM
;
Yong Chul BAE
;
Mae Ja PARK
Author Information
1. Department of Anatomy, School of Medicine, Kyungpook National University, Daegu, Korea. mjpark@knu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
embryonic development;
endoderm;
fibroblast growth factors;
SU 5402;
Xenopus
- MeSH:
Animals;
Endoderm/drug effects/embryology/*metabolism;
Fibroblast Growth Factors/antagonists & inhibitors/genetics/*physiology;
Gene Expression Regulation, Developmental/drug effects;
In Situ Hybridization;
Pyrroles/administration & dosage/pharmacology;
Receptors, Fibroblast Growth Factor/genetics/physiology;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction/drug effects;
Xenopus Proteins/antagonists & inhibitors/genetics/*physiology;
Xenopus laevis/embryology/genetics/*physiology
- From:Experimental & Molecular Medicine
2008;40(5):550-557
- CountryRepublic of Korea
- Language:English
-
Abstract:
We have previously shown that the inhibition of fibroblast growth factor (FGF) signaling induced endodermal gene expression in the animal cap and caused the expansion of the endodermal mass in Xenopus embryos. However, we still do not know whether or not the alteration of FGF signaling controls embryonic cell fate, or when FGF signal blocking is required for endoderm formation in Xenopus. Here, we show that FGF signal blocking in embryonic cells causes their descendants to move into the endodermal region and to express endodermal genes. It is also interesting that blocking FGF signaling between fertilization and embryonic stage 10.5 promotes endoderm formation, but persistent FGF signaling blocking after stage 10.5 restricts endoderm formation and differentiation.
