Study on fingerprint of Zhishi-Xiebai-Guizhi Decoction by HPLC and determination of 10 index components
10.7501/j.issn.0253-2670.2020.09.020
- VernacularTitle: 枳实薤白桂枝汤HPLC指纹图谱及10种指标成分含量测定研究
- Author:
Hai-Jian YUAN
1
Author Information
1. Taizhou Polytechnic College
- Publication Type:Journal Article
- Keywords:
Adenosine;
Chemometrics analysis;
Cinnamic acid;
Coumarin;
Fingerprint;
Hesperidin;
Honokiol;
HPLC;
Magnolol;
Naringin;
Neohesperidin;
Principal component analysis;
Quality control;
Quality evaluation;
Quercetin;
Synephrine;
Zhishi Xiebai Guizhi Decoction
- From:
Chinese Traditional and Herbal Drugs
2020;51(9):2448-2459
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC fingerprint analysis method of Zhishi Xiebai Guizhi Decoction (ZXGD) and the content determination method of 10 index components of the decoction, and carry out the relevant evaluation and analysis. Methods: HPLC method was used to establish the fingerprint analysis method of ZXGD and then similarity evaluation was carried out. Ten index components of the decoction were determined, the influence of the change of compatibility of medicinal materials on the content of index components was analyzed. Cluster analysis and other chemometrics methods were used to analyze the relevant data and evaluate the impact and value of quality control related indicators of ZXGD. Results: The similarity of 10 batches of samples ranged from 0.376 to 0.990, and that of five batches was more than 0.9, which indicated that the similarity of 10 batches of samples was quite different. In 10 batches of samples, S1, S2, S3, S5, S6, S8 and S10 were in one group, S4 and S9 were in one group, S7 was in one group. In this study, 30 characteristic peaks were calibrated, and principal components 1-6 was the main factor affecting the quality evaluation of medicinal materials. Among the 30 characteristic peaks, peak 21 (neohesperidin), 26, 29 (honokiol), 3, 23, 17, 30 (magnolol), 5, 24 (coumarin), 28, and 7 were the key components. The results showed that except quercetin, the other nine components had good linear relationship, precision, stability and repeatability in the range of mass concentration. Different compatibilities could increase or inhibit the dissolution of related components in medicinal materials. Conclusion: The HPLC method can be used for the simultaneous determination of ten chemical components in ZXGD. The method is efficient, accurate and reproducible, and can be used for the quality control and evaluation of ZXGD.
