Effects of different decoction methods on functional components of rhubarb based on pseudo-targeted metabolomics
10.7501/j.issn.0253-2670.2020.10.017
- VernacularTitle: 基于拟靶标代谢组学考察不同煎煮方式对大黄功效组分煎出的影响
- Author:
Wen-Juan ZHOU
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Anthraquinone glycosides;
Chemical components;
Clearing heat and detoxification;
Dianthrone glycosides;
Different decoction methods;
Efficacy;
LTQ-Orbitrap-MSn;
MRM;
Multivariate statistical analysis;
Pseudo-targeted metabolomics;
Purgation;
QQQ-MS;
Rhubarb;
Tannins
- From:
Chinese Traditional and Herbal Drugs
2020;51(10):2755-2766
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The effects of different decoction time and different decoction temperature on its chemical constituents of anthraquinones, anthrone, and tannins were revealed by pseudo-targeted metabolomics, which provided the basis for clinical use of rhubarb. Methods: Using LTQ-Orbitrap-MSn to accurately excavate the characteristic ions of various chemical components of rhubarb, the ion pairs of chemical components were determined as much as possible by characteristic ions to obtain the peak area of various components by using the MRM mode of QQQ-MS. The effects of different decoction methods on the chemical components of rhubarb was compared by multivariate statistical analysis combined with paired t test. Results: Both decoction time and decoction temperature have impacts on the chemical components of rhubarb decoction. Short-term decocting for 15 min was beneficial to the dissolution of dianthrone glycosides and anthraquinone glycosides, while long-term decocting for 60 min was beneficial to the dissolution of tannins; Compared with boiling water maceration, boiling water decoction was more favorable for the dissolution of anthraquinones and tannins. Conclusion: This paper adopts the method of pseudo-targeted metabolomics, and clearly points out that short-term decocting or maceration is conducive to exerting the laxative effect of rhubarb, and long-time boiling decocting is conducive to exerting clearing heat and detoxifying efficacy, which provides a reference for the clinical application of rhubarb.
