Rapid analysis of chemical constituents of Huoxue Zhitong Capsules based on UPLC-Q-TOF/MS
10.7501/j.issn.0253-2670.2020.12.004
- VernacularTitle: 基于UPLC-Q-TOF/MS技术活血止痛胶囊化学成分的快速分析
- Author:
Ji-Hong ZHANG
1
Author Information
1. Tianjin University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Benzoquinones;
Huoxue Zhitong Capsule;
Saponins;
Terpenoids;
UPLC-Q-TOF/MS
- From:
Chinese Traditional and Herbal Drugs
2020;51(12):3139-3146
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To rapidly analyze the chemical constituents of Huoxue Zhitong Capsules performed by ultra-high performance liquid chromatography quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Methods: A Waters Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) was used with 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile as a mobile phase. The flow rate was 0.4 mL/min; The samples were collected in positive and negative ion modes, respectively. The processed data was analyzed by Masslynx 4.1TM software. Results: A total of 113 compounds were identified in positive and negative ion mode, including terpenoids, saponins, organic acids, benzoquinones and others, and the medicinal sources of the compounds were assigned. Conclusion: The established method can systematically, quickly and accurately identify various chemical components of Huoxue Zhitong Capsule. This study provides a reference for the selection of quality evaluation indicators and the basis of pharmacodynamics of Huoxue Zhitong Capsule.
