Study on intervention of triptolide on metabolism of LC/IT-TOF-MS in ulcerative colitis
10.7501/j.issn.0253-2670.2020.13.017
- VernacularTitle: 基于LC/IT-TOF-MS技术的雷公藤甲素干预溃疡性结肠炎的代谢组学研究
- Author:
Guang-Chao MA
1
Author Information
1. Yunnan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Arginine;
Bile acid;
Differential metabolites;
Metabolic pathways;
Proline;
Serum;
Triptolide;
Ulcerative colitis
- From:
Chinese Traditional and Herbal Drugs
2020;51(13):3493-3500
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The effect of triptolide (TP) on endogenous metabolites in mice with ulcerative colitis (UC) was analyzed by means of metabolomics, and the metabolic pathway and possible mechanism of TP in UC were discussed. Methods: C57BL/6 mice were randomly divided into blank control group, model group, and triptolide group. Dextran sulfate (DSS) was used to induce UC mice model. The serum samples of mice were detected by high performance liquid chromatography-mass spectrometry and characterized by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to identify the potential biomarkers and possible metabolic pathways. Results: Compared with the blank control group, a total of 15 potential biomarkers, such as cholic acid, bezoar cholic acid, goose-deoxycholic acid, citrulline, guanidine butyric acid, aminoacetic acid, and cis-aconitic acid, were found and identified in serum. Compared with the model group, the potential biomarkers showed a tendency of callback to normal level after TP intervention. Conclusion: Metabolomics analysis reveals that TP had certain therapeutic effects on UC mice, and its mechanism may be related to regulating primary bile acid biosynthesis, arginine, and proline metabolism.
