Content determination of five flavonoids in Abelmoschus esculentus from different habitats by HPLC
10.7501/j.issn.0253-2670.2020.14.026
- VernacularTitle: 不同产地黄秋葵中5种黄酮成分HPLC分析
- Author:
Yun TANG
1
Author Information
1. Jiangxi Key Laboratory of Natural Active Pharmaceutical Ingredients, Yichun University
- Publication Type:Journal Article
- Keywords:
5,7,4'-trihydroxyflavone;
Abelmoschus esculentus (Linn.) Moench;
Flavonoids;
HPLC;
Isoquercitrin;
Quercetin;
Quercetin-3-O-gentiobioside;
Quercetin-3-O-rutinoside
- From:
Chinese Traditional and Herbal Drugs
2020;51(14):3797-3801
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determination of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin, and 5,7,4'-trihydroxyflavone in Abelmoschus esculentus from 12 different regions of China. Methods: COSMOSIL column (250 mm × 4.6 mm, 5.4 μm) was used. The gradient elution was carried out with acetonitrile and 0.2% phosphoric acid aqueous solution. The detection wavelength was 353 nm and the flow rate was 1 mL/min. Results: The contents of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin and 5,7,4'-trihydroxyflavone had good linear relationship in the ranges of 15.72- 251.50 μg/mL (r = 0.999 5), 15.47-247.50 μg/mL (r = 0.999 6), 14.41-230.50 μg/mL (r = 0.999 5), 21.88-350.00 μg/mL (r = 0.999 7), and 17.25-276.00 μg/mL (r = 0.999 1). The average sample recovery rate was 97.46%, 96.74%, 100.21%, 95.66%, 98.35%, and RSD was 1.97%, 1.37%, 1.86%, 2.72%, 2.23%, respectively. Conclusion: This method is simple and accurate. It is suitable for simultaneous determination of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin and 5,7,4'-trihydroxyflavone. The content of the five flavones can be used as one of the methods for evaluating the quality of A. esculentus.
