Toxicity and mechanisms of celastrol on human biliary epithelial cells
10.7501/j.issn.0253-2670.2020.14.013
- VernacularTitle: 雷公藤红素对胆管细胞的毒性作用及机制研究
- Author:
Ya-Jing LI
1
Author Information
1. School of Life Sciences, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Celastrol;
Cholangiocyte toxicity;
Human biliary epithelial cells;
Proliferation
- From:
Chinese Traditional and Herbal Drugs
2020;51(14):3700-3707
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the toxicity and mechanisms of celastrol (CEL) on human biliary epithelial cells. Methods: The effects of CEL on cell morphology and cell viability changes were observed by CCK-8 experiment and microscope. Cell scratch experiment was used to detect the effect of CEL on cell migration. The effects of CEL on cell cycle and cell apoptosis were detected by flow cytometry. The mRNA and protein expression of apoptosis-related genes Caspase-3, Bax and Bcl-2 were detected by qRT-PCR and Western blotting. Results: CEL inhibited cell proliferation and changed cell morphology at 400-2 000 nmol/L. At 200-800 nmol/L, cell migration was inhibited. At 800-1 200 nmol/L, G0/G1 phase was arrested. At 400-1 200 nmol/L, cell apoptosis was induced and the expression of apoptosis-related genes was increased. Conclusion: CEL showed cholangiocyte toxicity through affecting cell viability, cell migration, preventing cell cycle and promoting cell apoptosis of human biliary epithelial cells.
