Optimization of water extracting and refining procession of Sijunzi Decoction Granules by orthogonal design and pharmacodynamics test
10.7501/j.issn.0253-2670.2020.16.012
- VernacularTitle: 正交设计结合药效学实验优选四君子汤颗粒水提及精制工艺
- Author:
Fang-Hong SHANG
1
Author Information
1. Chongqing Academy of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
Ginseng Radix et Rhizoma;
Glycyrrhizae Radix et Rhizoma;
Multi-index evaluation;
Orthogonal design;
Refine;
Sijunzi Decoction Granules;
Spleen deficiency syndrome;
Water extracting procession
- From:
Chinese Traditional and Herbal Drugs
2020;51(16):4191-4200
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the water extracting and refining procession of Sijunzi Decoction Granules (SDG). Methods: The orthogonal test method was used to study the four factors including the soak time, amount of water added, extraction time and frequency by taking the dry extract yield of the medicinal materials and contents of ginsenoside Rg1, Re, Rb1, glycyrrhizic acid, and liquiritin as indicators to optimize the water extracting process of SDG. To optimize the alcohol precipitation process of SDG, the factors including the concentration of the medicinal materials, alcohol content, and time of alcohol precipitation were investigated. The extracts before and after alcohol precipitation were compared by intervening spleen deficiency syndrome pharmacodynamics experiment. Results: The best water extracting procession of SDG was soaked for 60 min with 10 times of water, decocted three times, 30 min for each time. The optimal water extraction by alcohol sedimentation process was to concentrate the filtrate of water extraction to 1 mL, which was equivalent to 0.5 g of the original medicinal materials, with 80% alcohol content and 12 h alcohol precipitation time. Compared with the model group, the activity of salivary amylase and the conent of serum gastrin in the water extracting group and water extracting by alcohol sedimentation group of SDG were significantly increased and could improve the absorption function of xylose in small intestine of rats with spleen deficiency syndrome (P < 0.05); Compared with the water extracting group of SDG, there was a significant difference in D-xylose excretion rate in the water extracting by alcohol sedimentation group of SDG (P < 0.05), but there was no significant difference in activity of salivary amylase and content of serum gastrin (P > 0.05). Conclusion: The alcohol precipitation process is not suitable for refining of SDG. So the water extracting process is finally selected as the best extraction process of SDG.
