Isolation and activity evaluation of endophytic fungi from Glycyrrhiza uralensis
10.7501/j.issn.0253-2670.2020.17.023
- VernacularTitle: 乌拉尔甘草内生真菌分离及活性初探
- Author:
Ming-Jun YANG
1
Author Information
1. School of Life Science and Engineering, Lanzhou University of Technology
- Publication Type:Journal Article
- Keywords:
Alpha-glucosidase inhibited activity;
Antibacterial activity;
Antioxidant activity;
Endophytic fungi;
Glycyrrhiza uralensis Fisch
- From:
Chinese Traditional and Herbal Drugs
2020;51(17):4538-4546
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate and purify endophytic fungus from Glycyrrhiza uralensis, and screen the specific strain with better antibacterial and antioxidant activity (DPPH• radical scavenging, total reducing power, determination of hydroxyl radical scavenging) and anti-alpha-glucosidase activity. Methods: The endophytic fungi were isolated and purified from G. uralensis by tissue cutting. N-butanol, ethyl acetate and ethanol were used to extract the fermentation liquid and mycelium. Antibacterial activity was detected by filter paper. Three methods were used to characterize antioxidant activity. A total of 36 endophytic fungi were isolated from G. uralensis by PNPG method. Results: A total of seven genera were isolated from 108 samples by concentrated fermentation liquid extraction. Trichoderma. sp was the dominant species. The experimental results showed that 43.52% of the samples had different degrees of antibacterial effect, of which 8.33% performed well; The extracts showed different levels of antioxidant activity, of which 4.63% to 10.12% showed better performance in the three methods. 99.07% of the samples had different levels of anti-α-glucosidase activity, of which 5.56% of the samples performed well. Conclusion: The strains isolated from G. uralensis have good antibacterial activity, antioxidant activity, and anti-alpha-glucosidase activity, which further study is needed.
