Mechanism and experimental verification of antioxidant action of Heyan Kuntai Capsule based on systematic pharmacology
10.7501/j.issn.0253-2670.2020.17.019
- VernacularTitle: 基于系统药理学的和颜®坤泰胶囊发挥抗氧化作用的机制及其实验验证
- Author:
Yin-Mei CHEN
1
Author Information
1. Liupanshui Municipal Maternity and Infant Hospital of Guizhou
- Publication Type:Journal Article
- Keywords:
Bioinformatics analysis;
Heyan Kuntai Capsule;
In vitro experiment;
Mitochondrial function;
Oxidative stress;
Signaling pathways
- From:
Chinese Traditional and Herbal Drugs
2020;51(17):4506-4512
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore and verify the potential antioxidation mechanism of traditional Chinese medicine Heyan Kuntai Capsule (HKC). Methods: Gene targets of 21 ingredients of HKC were searched for through Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and analysis platform, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis with Database for Annotation, Visualization and Integrated Discovery (DAVID) tools. Further, the anti-oxidative function of HKC was validated in an in vitro H2O2-induced oxidative stress model in MII oocytes. Results: A total of 151 genes were obtained as targets to 21 components of HKC, whose GO were further significantly enriched in mitochondrion, superoxide dismutase activity and energy pathways, while KEGG showed the enrichment in PI3K/AKT, mTOR and ErbB1 signaling pathways. Afterwards, it was confirmed that HKC increased ATP production in H2O2-treated MII oocytes by improving mitochondrial function and reduced the dissipation of mitochondrial membrane potential (ΔΨm) to exert the antioxidation effect. Conclusion:s HKC exerts anti-oxidative effect by improving mitochondrial function.
