Inhibitory effects of iridoid glycosides from Boschniakia rossica combined with 5-fluorouracil on human hepatoma HepG2 and SK-Hep1 cells epithelial-mesenchymal transition
10.7501/j.issn.0253-2670.2020.17.018
- VernacularTitle: 草苁蓉环烯醚萜苷联合5-氟尿嘧啶对人肝癌HepG2SK-Hep1细胞上皮间质转化的抑制效果研究
- Author:
Xue-Hua DONG
1
Author Information
1. Affiliated Hospital of Yanbian University
- Publication Type:Journal Article
- Keywords:
5-FU;
Boschniakia rossica Fedtsch. et Flerov;
Cell adhesion rate;
Cell survival rate;
E-cadherin;
Epithelial-mesenchymal transition;
Hepatocellular carcinoma;
HepG2 cells;
Immunofluorescence;
Iridoid glycosides;
Matrix metalloproteinase;
SK-Hep1 cells;
Synergistic effect;
TGF-β1;
Vimentin;
Western blotting
- From:
Chinese Traditional and Herbal Drugs
2020;51(17):4498-4505
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the inhibitory effect of iridoid glycosides from Boschniakia rossica (IGBR) combined with 5-Fu on epithelial-mesenchymal transition induced by TGF-β1 in human hepatoma SK-Hep1 and HepG2 cells, and compare the efficacy of drugs. Methods: The survival ability of HepG2 and SK-Hep1 cells was detected by MTT and the combination index (Q value) was calculated to judge the interaction of combined drugs. The EMT model of HepG2 and SK-Hep1 cells was established. The cell adhesion rate was detected by MTT. The expression of matrix metalloproteinase (MMP) MMP2, MMP7, MMP9, Snail, and Slug was detected by Western blotting. The localization and expression intensity of E-cadherin and Vimentin was detected by immunofluorescence. Results: MTT showed that compared with the control group, the 5-FU group, IGBR group and combination group cell survival ability were decreased (P < 0.05) at 48 h after administration; IGBR and 5-Fu had an additive or synergistic effect. Compared with the model group, the adhesion rate of 5-FU group, IGBR group and combination group was reduced (P < 0.05). Western blotting results showed that compared with the control group, the expression of MMP2, MMP7, MMP9, Snail, Slug were up-regulated (P < 0.05) in the model group. Compared with the model group, the expression of MMP2, MMP7, MMP9, Snail and Slug were down-regulated (P < 0.05) in 5-FU group, IGBR group and combination group. Compared with the control group, immunofluorescence showed that the E-cadherin fluorescence intensity was decreased in the model group, but the Vimentin fluorescence intensity was increased. Compared with the model group, the E-cadherin fluorescence intensity was increased in 5-FU group, IGBR group and combined group, but the Vimentin fluorescence intensity was decreased. Conclusion: IGBR and 5-Fu can inhibit human hepatoma EMT. The combined drugs have the combined effect on HepG2 cells and synergistic effect on SK-Hep1 cells. The therapeutic effect on SK-Hep1 cells is better than HepG2 cells.
