MSAP analysis of DNA methylation between pogoston-type and patchouliol-type of Pogostemon cablin
10.7501/j.issn.0253-2670.2020.20.023
- VernacularTitle: 醇型和酮型广藿香DNA甲基化的MSAP分析
- Author:
Yi-Jie XIE
1
Author Information
1. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
DNA methylation;
MSAP;
Patchouliol-type of Pogostemon cablin;
Pogostemon cablin (Blanco) Benth.;
Pogoston-type of Pogostemon cablin
- From:
Chinese Traditional and Herbal Drugs
2020;51(20):5293-5301
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the DNA methylations between two chemical types of Pogostemon cablin using MSAP technique. Methods: Methylation sensitive amplification polymorphism (MSAP) technique was used to detect the DNA methylation degree of two chemical type and a total of 197 P. cablin samples. Results: The highest or higher values of the Shannon polymorphism index of the four types of MSAP locus information were produced in the five areas of Yangchun, Deqing, Gaozhou Datong, Lubu, and Guangning. It formed two branches with P. cablin Shipaiensis (Pogostone-type) and the rest (Patchouliol-type). The percentage of variation among the populations was 60.66%, which was far greater than that within the population. A total of 10 differential fragments were screened out, sequenced and analyzed, one of those belongs to the serine/threonine protein kinase family. Conclusion: The MSAP technique can be used to identify the different origins of P. cablin. The formation of different chemical types of P. cablinis closely related to its DNA methylation level, and further research is needed.
