Construction of aseptic cultural system of Salvia miltiorrhiza and determination of active components by HPLC analysis
10.7501/j.issn.0253-2670.2020.22.021
- VernacularTitle: 丹参无菌培养体系构建及有效成分含量的HPLC分析
- Author:
Xiao-Bin HEI
1
Author Information
1. Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Caffeic acid;
Callus;
Danshensu;
Ferulic acid;
Regenerated plantlet;
Rosemary acid;
Salvia miltiorrhiza Bge.;
Salvianolic acid A;
Salvianolic acid B;
Salvianolic acid B;
Tanshinone IIA
- From:
Chinese Traditional and Herbal Drugs
2020;51(22):5833-5838
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The regeneration system of Salvia miltiorrhiza was constructed and contents of active components of the sterile materials were determined, so as to provide the basis for the rapid propagation and secondary metabolism regulation of S. miltiorrhiza. Methods: The optimum hormone ratio for inducing callus was screened by orthogonal test. The buds and roots induction were conducted to estabilish the tissue culture system. The contents of effective components were evaluated by HPLC analysis. Results: The suitable medium for callus induction was MS+6-BA (2.0 mg/L)+NAA (1.0 mg/L)+2,4-D (0.5 mg/L). The preferred enrichment medium of adventitious bud induction was MS+6-BA (2.0 mg/L)+NAA (1.0 mg/L). And rooting medium was 1/2 MS+NAA (0.5 mg/L). Seven active components in aseptic seedlings, callus, and regenerated seedlings could be detected with significant differences in different aseptic materials (P < 0.05). The contents of rosmarinic acid and salvianolic acid B were higher than the others. Conclusion The culture system of S. miltiorrhiza was successfully established, and vigor regenerated seedlings were also obtained. The accumulation of active components in the three sterile materials showed difference, laying a foundation for further study in S. miltiorrhiza.
