Anti-inflammatory effect of impressic acid from Acanthopanax gracilistylus based on NF-κB signaling pathway
10.7501/j.issn.0253-2670.2020.22.016
- VernacularTitle: 基于NF-κB信号通路的细柱五加中impressic acid的抗炎作用研究
- Author:
Jin XIAO
1
Author Information
1. School of Pharmacy, Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Acanthopanax gracilistylus W. W. Smith;
Anti-inflammatory;
High-mobility group box 1 protein;
Impressic acid;
Nuclear factor-κB;
Triterpene
- From:
Chinese Traditional and Herbal Drugs
2020;51(22):5781-5787
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the anti-inflammatory effect of impressic acid (IA) from Acanthopanax gracilistylus on lipopolysaccharide (LPS)-induced RAW264.7 cells. Methods: RAW264.7 cells were stimulated by LPS to establish an inflammatory model. The cytotoxicity of IA on RAW 264.7 cells was detected by EZ4U cell proliferation and cytotoxicity analysis kit. The level of nitric oxide (NO) was determined by Griess. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were determined by ELISA. The expressions of TNF-α and IL-1β mRNA were detected by RT-PCR. The expression of high-mobility group box 1 protein (HMGB1) was detected by western blotting. The levels of nuclear factor-κB (NF-κB) in cytoplasm and nucleus were measured by ELISA. Results: IA significantly suppressed the levels of TNF-α and IL-1β, the expression of HMGB1 protein, and the translocation of NF-κB from cytoplasm to nucleus in LPS-induced RAW264.7 cells (P < 0.05, 0.01). Conclusion IA from A. gracilistylus has an anti-inflammatory effect on LPS-induced RAW264.7 cells.
