Analysis and Identification of Sesquiterpenes in Alpinia oxzphylla Miq Based on UPLC-Q-Orbitrap HRMS
10.7501/j.issn.0253-2670.2020.24.005
- VernacularTitle: 基于UPLC-Q-Orbitrap HRMS益智仁中倍半萜类物质的分析与鉴定
- Author:
Meng-Ya ZHANG
1
Author Information
1. Department of Pharmacy, First Affiliated Hospital of Zhengzhou University
- Publication Type:Journal Article
- Keywords:
Alpinia oxyphylla Miq;
Extraction process;
Sesquiterpenes;
UPLC-Q-Orbitrap HRMS
- From:
Chinese Traditional and Herbal Drugs
2020;51(24):6168-6177
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a rapid and accurate analytical method for the identification of complex system of traditional Chinese medicine, and to systematically clarify the chemical composition of sesquiterpenes in Alpinia oxyphylla. Method: On the basis of optimizing the extraction process of sesquiterpenes, the accurate molecular weight and secondary fragment ions information of unknown compounds were captured by ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). Compared with the relative retention time and mass spectrometry data of the reference substance, combined with relevant references and databases, the sesquiterpene unknown compounds in the fruits of A. oxyphylla were accurately and rapidly characterized. Results: A total of 24 sesquiterpenes were identified and classified into four categories according to their skeleton structure, including nine eudesmane-type, six cadinane-type, eight eremophilane-type, and one oplopanone-type. Conclusion: In this study, the established analytical method was used to realize the rapid and accurate identification of sesquiterpenes in the fruits of A. oxyphylla, which provided a theoretical basis for the research on the pharmacodynamic substance basis and quality control of the fruits of A. oxyphylla.
