Determination of the content of rocuronium bromide by HPLC
- Author:
Geng-Shen SONG
1
Author Information
1. Hebei Institute for Drug Control
- Publication Type:Journal Article
- Keywords:
Content assay;
HPLC;
Rocuronium bromide
- From:
Journal of International Pharmaceutical Research
2010;37(2):127-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a HPLC method to determinate the content of rocuronium bromide. Methods: NH2-bonding silica gel was used as the stationary phase. The mobile phase consisted of 0.04 mol/L tetramethyl ammonium hydroxide (pH adjusted to 7.4 by phosphoric acid) and acetonitrile (10:90). The column temperature was 35°C. The detection wavelength was 207 nm. Results: The relation between concentration and peak area was linear in the range of 0.1251-2.0065 mg/ml with r2 of 0.9999. The average recovery rate was 99.9% with RSD of 0.1%. Conclusion: This method is exclusive, sensitive and is reliable for the determination of rocuronium bromide.
