Dtermination of tetramethylpyrazine in Curcuma aromatic Salisb at different storage time by HPLC
10.13220/j.cnki.jipr.2014.05.019
- Author:
Li LI
1
Author Information
1. Henan Medical Technician Institute
- Publication Type:Journal Article
- Keywords:
Curcuma aromatic Salisb;
HPLC;
Tetramethylpyrazine
- From:
Journal of International Pharmaceutical Research
2014;41(5):603-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a determining method for tetramethylpyrazine in Curcuma aromatic Salisb by high-perfomance liquid chromatography. Methods: Tetramethylpyrazine was extracted from Curcuma aromatic Salisb by methanol. Kromasil C18 (250 mm×4.6 mm, 5μm) was used as stationary phase, methanol: 0.8 % sodium acetate solution (42∶58) was used as mobile phase, the flow rate was 1 ml/min and the wavelength of detection was at 282 nm. Results: The calibration curve showed good linearity within the concentration range of 220 ng, r=0.9999, and the average recovery was 99.56%, RSD=0.65%. Conclusion: The method is simple, quick and suitable for the determination of tetramethylpyrazine in Curcuma aromatic Salisb. No literature home and abroad has yet reported quality control of Curcuma aromatic Salisb by tetramethyl pyrazine.
