Simultaneous determination of gallic acid, methyl gallate and ellagic acid in gall of Quercus infectoria Olivier by HPLC

Author: Liang-Miao OU ¹
Author Information

1. College of TCM, Xinjiang Medical University
Publication Type:Journal Article
Keywords: Ellagic acid; HPLC; Methyl gallate; Quercus infectoria Olivien gallic acid
From: Journal of International Pharmaceutical Research 2015;42(5):637-641
CountryChina
Language:Chinese
Abstract: Objective To dertermine the content of gallic acid, methyl gallate and ellagic acid in galls of Quercus infectoria Olivier from different batches by HPLC. Methods The chromatogram colume was Agilent Zorbax SB-C18 (4.6 mm x 250 mm, 5 μm), gradiently eluting with methanol as mobile phase A and 0.01% phosphoric acid as B at a flow rate of 1 ml/min. The injection volumn was 5 μl. Detection wavelength was at 258 nm. Results Gallic acid was linear in the range of 0.0318-0.1871 g/L with correlation coefficient of 0.9994. The average recovery was 97.52% with RSD 1.41%. Methyl gallate was linear in the range of 0.0769-0.4612 g/L with correlation coefficient of 0.9994. The average recovery was 99.15% with RSD at 1.46%. Ellagic acid was linear in the range of 0.0158-0.0553 g/L with correlation coefficient of 0.9998. The average recovery was 102.75% with RSD at 0.87%. Conclusion The method is convenient, fast, accurate and practicable, and can be used for controling the quality of the galls of Q. infectoria.