HPLC fingerprint of analgesic ingredients in cangbaiqutong capsules
10.13220/j.cnki.jipr.2015.04.016
- Author:
Zhong JIA
1
Author Information
1. Gansu Agricultual University
- Publication Type:Journal Article
- Keywords:
Berberine hydrochloride;
Cangbaiqutong capsules;
Gentiopicroside;
Paeoniflorin;
Phellodendrine chloride;
Tetrandrine
- From:
Journal of International Pharmaceutical Research
2015;42(4):519-523
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish fingerprint of analgesic ingredients in Cangbaiqutong capsules by HPLC, and control different production batches of Cangbaiqutong capsules’ quality. Methods Waters XSELECT CSH-C18 (4.6 mmxl50 mm, 5.0μm) chromatographic column was used and eluted with acetonitrile -0.1% phosphoric acid solution gradiently in the flow rate of 1.0 ml/ min. The detection wavelength was 284 nm with column temperature of 30°C. rrith phellodendrine chloride, gentiopicroside, paeoniflorin, tetrandrine and berberine hydrochloride as the object references, ten batches of Cangbaiqutong capsules were tested and analyzed by similarity comparison. Results fingerprint spectrum in Cangbaiqutong capsules had 20 common peaks in total, and characteristic spectra of Cortex Phellodendri, Gentiana, Radix Paeoniae Rubra and Radix Stephaniae Tetrandrae were found, while similarity of HPLC fingerprint was more than 0.9 among those batches of samples. Conclusion Using HPLC fingerprint can be used to evaluate the Cangbaiqutong capsules% quality in the round, which could improve the quality control standard in productive process of Cangbaiqutong capsules.
