Protective effect of amentoflavone on radiation-reduced damage in mice bone marrow
10.13220/j.cnki.jipr.2015.03.016
- Author:
Xiao-Juan ZHANG
1
Author Information
1. Medical School of Qinghai University
- Publication Type:Journal Article
- Keywords:
Amentoflavone;
Bone marrow cells;
Radiation injures;
Radiation protection
- From:
Journal of International Pharmaceutical Research
2015;42(3):375-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and possible mechanism of amentoflavone (AF) on bone marrow cells of mice injured by irradiation. Methods Primary bone marrow cells of male C57BL/6 mice were cultured and randomly divided into 4 groups (normal control, radiation control,AF 2.5 μmol/L and 5 μmol/L), with 3 samples in each group. After treated with AF for 12 h, the cells were injured by 12 Gy60Co γ irradiation. 6 h and 12 h post-irradiation, apoptosis was evaluated by using Hoechst 33258 stain, cell cycle was determined by flow cytometry while the level of TNF-α was tested by ELISA. Results The cell cycle arrest and apoptosis were not significantly affected by Amentoflavone. Amentoflavone 5 μmol L) could significantly inhibit the production of TNF- α on cell supernatant of mouse bone marrow cells at 6 h or 12 h after radiation and 2.5 μmol/L Amentoflavone could significantly inhibit the production of TNF- α at 6 h after radiation. Conclusion Taken together, the data suggest that AF may have radioprotection against damage in mice bone marrow by inhibiting the production of TNF-α.
