Influence factors of microdialysis linear probe recovery for in vivo and in vitro cyclophosphamide calibration by HPLC-MS/MS
10.13220/j.cnki.jipr.2015.03.013
- Author:
Chun-Sheng LIU
1
Author Information
1. The Second Artillery General Hospital
- Publication Type:Journal Article
- Keywords:
Calibration;
Cyclophosphamide;
HPLC-MS/MS;
Linear probe;
Microdialysis
- From:
Journal of International Pharmaceutical Research
2015;42(3):362-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an optimal linear probe recovery correction method for in vivo and in vitro cyclophosphamide. Methods with ringer solution as the perfusion, retrodialysis gain and loss method were used to calibrate the linear probe. The impact of different perfusion flow rates on microdialysis relative recovery for cyclophosphamide was investigated by HPLC-MS/MS. Results When the perfusion was 1.0μl/min, in vitro recovery results were in the range of (24.11±1.75)% to (50.93±0.91)% for the gain method, and (26.19 ±0.76)% to(53.31 ±1.23)% for the loss method; recovery results in vivo were (26.19±0.76)%. Conclusion Cyclophosphamide is suitable for microdialysis sampling. This experiment calibration method is simple and effective.
