Preliminary evaluation of 10-hydroxycamptothecin loaded human serum albumin nanoparticles prepared by drug-liquid compound method
10.13220/j.cnki.jipr.2015.03.012
- Author:
Zhen-Bo YANG
1
Author Information
1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences
- Publication Type:Journal Article
- Keywords:
10-hydroxycamptothecin;
Drug liquid compound;
Entrapment efficiency;
Human serum albumin based nanoparticles;
Polyethylene glycol
- From:
Journal of International Pharmaceutical Research
2015;42(3):355-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize and evaluate in vitro and in vivo of the 10-hydroxycamptothecin (HCPT) loaded human serum albumin nanoparticle (HSA-NP) prepared by drug-liquid compound method. Methods The HCPT-HSA-NP was prepared with low weight polyethylene glycol drug-liquid compound and blank albumin nanoparticle. Then the in vitro evaluations were conducted with tests of entrapment efficiency, solution stability, accumulative release, morphous investigation and X-ray powder diffraction. At the same time, the primary pharmacodynamics comparison between HCPT injection and the nano preparation-8 mg/kg) was carried out on animal tumor model. Results The obtained HCPT-HSA-NP fitted to the basal features of nano preparation. The entrapment efficiency was averagely higher than 99% for each sample and the solution was stable. In vitro accumulative release study showed that the preparation had long-term release pattern over 100 hours. In vivo pharmacodynamics study showed that the HCPT-HSA-NP was significantly more effective than HCPT injection (P<0.01). Conclusion The drug-liquid compound method can be used to prepare HCPT-HSA-NP.
