Comparative study on three preparation methods of mouse postoperative ileus models
10.13220/j.cnki.jipr.2016.02.027
- Author:
Lin ZHANG
1
Author Information
1. Henan University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Inflammatory cells;
Intestinal propulsion rates;
Mucosa;
Postoperative ileus
- From:
Journal of International Pharmaceutical Research
2016;43(2):346-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective By observing the motility changes of the gastrointestinal tract in mouse postoperative ileus (POI) models prepared with three different methods, this study aims to compare the features of three types of models, and judge their suitability for drug evaluation. Methods Three methods were conducted to prepare the mouse POI models: air exposure, friction and extrusion. In the experiment, the gastric emptying rate, intestinal propulsive rate as well as the relatively percent dextran of blue-2000 in intestinal paragraphs were taken down to evaluate the motility of gastrointestinal tract. Meanwhile, the duodenum, jejunum, ileum and colon of mice were stained in HE method to observe the histopathological changes of each layer in mouse intestinal wall. Results When compared with the control group, the gastric emptying rate of the squeeze-only group was significantly suppressed (P < 0.01). The intestinal propulsive rate in the air exposure group, the friction group and the extrusion group were all suppressed, only the friction group and the extrusion group had statistical difference (P < 0.01). The relative percent of dextran blue-2000 in intestinal paragraphs in mice also show that the motility of the mouse gastrointestinal tract in both the friction and extrusion groups was significantly inhibited. Judging from the results of HE staining, the duodenums of mice in the air exposure group, friction group and extrusion group did not show much difference when compared with the control group. While for the jejunum, the intestinal mucosa and submucosa of mice in the squeeze group had a large quantity of hemorrhage and inflammatory cell infiltration. For the ileum and colon, the friction and extrusion groups showed different degrees of inflammatory cell infiltration, and the mice in the extrusion group suffered from ileal hemorrhage. Conclusion All the three preparation methods of mouse POI models can inhibit the intestinal movement of mice. The effect of the air exposure method causes the light injury. The extrusion method, which causes more serious injury, could be used in the study of the pathogenesis of postoperative ileus.The friction method that has a moderate damage to the intestine, is more suitable for drug screening and evaluation.
