Effects of Ento-I plastic on analgesia, anticoagulation and anti-thrombosis
10.13220/j.cnki.jipr.2016.03.020
- Author:
Rui FENG
1
Author Information
1. Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R and D, Dali University
- Publication Type:Journal Article
- Keywords:
Analgesia;
Anticoagulation;
Antithrombotic
- From:
Journal of International Pharmaceutical Research
2016;43(3):509-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the analgesic effect and the in vitro anticoagulation activity of Ento-I, along with its protective effect against the formation of collagen- adrenaline induced thrombus. Methods The threshold of pain in mice was deter mined with the method of hot plate. Mice thrombogenesis induced by collagen-epinephrine mixture injected through tail-vein was adopted for observing the mortality, hemiplegia recovery rates, hemiplegia formation time, survival time and time for hemiplegia recovery in mice to evaluate the protective effects of test drugs against thrombus in vivo. Anticoagulation effects of test drugs were detected by measuring the thrombin time (TT), prothrombin time (PT), and activated partial thromboplastin time (APTT) of rabbits with in vitro clotting model. Results The experiment of hot plate for analgesia showed that compared with those of normal control group Ento-I plastic could significantly prolong pain thresholds in mice at 30, 60 and 90 min after final administration (P<0.01). The mortality of collagen-epinephrine induced thrombotic hemiplegia was reduced while its recovery rates were significantly increased for mice administered Ento-I plastic (P<0.05). Compared with clopidogrel group, Ento-I plastic 2 mg/kg could effectively reduce the time for hemiplegia recovery (P<0.05). In addition, compared with substrate matrix group, Ento-I plastic (4.65 mg/kg) could significantly prolong TT, PT, and APTT in rabbit plasma (P<0.05, P<0.05 and P<0.01). Conclusion The Ento-I plastic possesses analgesic effect, anticoagulability and antithrombotic activity, and it may be a promising medicine for the treatment of thrombotic diseases.
