Simultaneous determination three kinds of components in Longze Xiongdan capsule by RP-HPLC
10.13220/j.cnki.jipr.2016.04.033
- 作者:
Cheng-Yi HAO
1
作者信息
1. College of Pharmacy, Jilin Medical University
- 出版类型:Journal Article
- 关键字:
Baicalin;
Berberine hydrochloride;
Gentiopicroside;
Longze Xiongdan capsule;
RP-HPLC
- 来源:
Journal of International Pharmaceutical Research
2016;43(4):757-760
- 国家China
- 语言:Chinese
-
摘要:
Objective To establish a method for simultaneous determination of gentiopicroside, berberine hydrochloride and baicalin in Longze Xiongdan capsule. Methods The separation was performed on a DIKMA Platisil ODS column (250 mm × 4. 6 mm, 5 µm) with gradient elution using methanol and 0.1% H3PO4 aqueous solution as mobile phase. The detection wavelength was set at 275 nm. Results Linear ranges of gentiopicroside, berberine hydrochloride and baicalin concentrations were 0.019-0.38 µg (r=0.9999), 0.15-3.00 µg (r=1.0000) and 0.078-1.56 µg (r=0.9997),respectively. Their average recoveries fell between 98.97% and 101.30%. Conclusion The method can be used as the standard for the quality control of Longze Xiongdan capsule.
