Development of HPLC-MS/MS method for determination of linezolid in human plasma

Author: Ming-Xing GUO ¹
Author Information

1. Department of Pharmaceutics, The General Hospital of the PLA Rocket Force
Publication Type:Journal Article
Keywords: HPLC-MS/MS; Linezolid; Plasma
From: Journal of International Pharmaceutical Research 2016;43(4):740-743
CountryChina
Language:Chinese
Abstract: Objective: To determine the concentration of linezolid in human plasma by high performance liquid chromatagraphy-tandem mass spectrometry(HPLC-MS/MS) so as to provide evidence for the rational administration in clinic. Methods: The plasma was extracted by acetonitrile with telmisartan as internal standard(IS). The mobile phase was 1 mmol/L ammonium acetate with 0.1% formic acid-acetonitrile with gradient elution. Quantitative analysis of [M+H]+ ions was m/z 338.1→m/z 296.3 (linezolid) and m/z 515.3→m/z 276.3 (telmisartan,the IS). Results: Excellent linear relationship was obtained from 0.23 to 46 µg/ml with correlation coefficient value of 0.9993. The lowest limit of quantitation was 46 ng/ml, the extraction recovery was from 87% to 109%. The intra- and inter- day precisions (relative standard deviations) ranged from 2.5% to 8.6%. Conclusion: The method is specific, sensitive and simple and can be used to determine the concentration of linezolid in clinic.