Determination of ropivacaine in rat plasma with liquid chromatography-tandem mass spectrometry
10.13220/j.cnki.jipr.2016.04.028
- Author:
Jie-Zhao ZHOU
1
Author Information
1. Wuhan Institute of Technology
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
Plasma;
Rat;
Ropivacaine
- From:
Journal of International Pharmaceutical Research
2016;43(4):736-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an LC-MS/MS method for the determination of ropivacaine in rat plasma. Methods: The chromatographic separation was achieved on an Agela MP C18(2.1 mm×50 mm,3 µm)analytical column. The multiple reaction monitoring(MRM) mode of the positive ion was adopted in the MS detection, and the precursors to the product ion transitions of m/z 275.2/126.1 and 326.0/291 were used to measure ropivacaine and internal standard (midazolam). Results: The method was linear over the ropivacaine concentration range of 0.5-250 ng/ml with the correlation coefficients r of 0.9946. The average inter-day precision values (RSD) were 1.32%-6.74%, the matrix effect values were 95.5%-102.6% and the average recovery values were 89.8%-104.7%. Conclusion: The analysis methods for ropivacaine are simple, accurate and sensitive, thus can be used for the pharmacokinetic research of ropivacaine incorporated cubic liquid crystal in rat.
