Determination of L-corydalmine in rat plasma by LC-MS/MS and the pharmacokinetic studies
10.13220/j.cnki.jipr.2016.05.031
- Author:
Shuai LIU
1
Author Information
1. Institute of Basic Medical Sciences
- Publication Type:Journal Article
- Keywords:
L-corydalmine;
LC-MS/MS;
Pharmacokinetics;
Rat
- From:
Journal of International Pharmaceutical Research
2016;43(5):971-974
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a simple, rapid and accurate analysis method for determination of L-corydalmine (L-CDL) in rat plasma. Methods The plasma sample was determined with propranolol as the internal standard (IS), the separation was accomplished in a Capcell PAK C18(2.1 mm×750 mm, 3 μm) column, and the mobile phase consisted of water (including 0.1% formic acid and 5 mmol/L ammonium formate) and acetonitrile (including 0.1% formic acid) at a flow rate of 0.25 ml/min. The mass spectrometer consisted of an ESI interface operating at positive ionization mode and the detection was performed using multiple reaction monitoring(MRM) at the transitions of m/z 342→192.1 for L-CDL and m/z 260→116.2 for propranolol. Method validation included the evaluation of the linearity range, lower LOQ, within-run and between-run precision, accuracy and stability, matrix effect and extraction recovery. Results The calibration curve was linear across the concentration range of 1-5000 ng/ml for L-CDL with a lower LOQ of 1 ng/ml. The within-run and between-run precision (RSD%) was in the range 0.1%-10%. The extraction recovery was in 93.5%-102.8% and the matrix effect for three QC was 108.3%-112.5%. L-CDL reached the peak concentration at 0.5 h after dosing. The main pharmacokinetic parameters of rats after igl administration were as follows: T1/2: (7.04±3.93)h, Cmax: (557.8±330.1)ng/ml, AUC0~24: (2408±630)h•ng/ml. Conclusion A simple, rapid, accurate, high sensitivity and repeatability method has been successfully developed, which can analyze the concentration of L-CDL in rat plasma. The method can be used for the investigation of pharmacokinetics of L-CDL in rats.
