Application of stable isotope dilution liquid chromatography-mass spectrometry in medical research
10.13220/j.cnki.jipr.2017.04.006
- Author:
Yu-Long GU
1
Author Information
1. Institute of Chinese Minority Traditional Medicine, Minzu University of China
- Publication Type:Journal Article
- Keywords:
Biomarker;
Drug;
Stable isotope dilution liquid chromatography-mass spectrometry(ID-LC-MS)
- From:
Journal of International Pharmaceutical Research
2017;44(4):326-331
- CountryChina
- Language:Chinese
-
Abstract:
Stable isotope dilution mass spectrometry(ID-MS)is a quantitative method for determining the content of unlabeled compounds with stable isotope labeled compounds as internal standard. Stable isotope dilution liquid chromatography-mass spectrometry(ID-LC-MS)has the advantages of high sensitivity, no quantitative separation of the analytes, and effective elimination of the biological matrices. The method has been widely used in quantitative analysis of biomarkers, drugs and their metabolites. In this paper, the mechanism and characteristics of ID-LC-MS are briefly introduced, and its medical applications are summarized.
