Optimization of the extraction process of main effective components in Lycii Fructus by central composite design-response surface method
10.13220/j.cnki.jipr.2019.05.008
- Author:
Zhi-Jia CUI
1
Author Information
1. School of Pharmaceutical Sciences, Gansu University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Central composite design;
Extraction process;
Lycii Fructus;
Response surface method
- From:
Journal of International Pharmaceutical Research
2019;46(5):375-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the extraction process of effective components in Lycii Fructus using central composite design(CCD)-response surface method(RSM). Methods: The yield of main effective components, polysaccharides, flavonoids, rutin and chlorogenic acid as well as the yield of total extract were used as indicator for evaluating the effect of extraction solvent, extraction time, solid- liquid ratio, extraction temperature and other factors. The single factor experiment was conducted to determine the range of various factor parameters, the factor design experiment was performed for the screening of the factors that had significant influence on the extraction efficiency of effective components, the maximum velocity rise experiment was carried out to determine the central value of the factors, and finally the optimal extraction process was optimized by the central composite design- response surface methodology. Results: The optimum extraction conditions of Lycii Fructus were obtained as follows: the extraction with 60% aqueous ethanol solution by ultrasound sonication at room temperature for 40.5 minutes, and the solid- liquid ratio was 1:27. Conclusion: The present optimal extraction process could effectively improve the extraction efficacy of effective components from Lycii Fructus, and the created CCD-RSM model and related conditions have good predictability and repeatability, which provides experimental and theoretical reference for further development and research of Lycii Fructus.
