Quality characterization of radix paeoniae alba in huangqi guizhi wuwu decoction
10.13220/j.cnki.jipr.2019.06.011
- Author:
Tian-Yi ZHANG
1
Author Information
1. Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Content determination;
Fingerprint chromatogram;
HPLC;
Paeonia lactiflora;
Radix Paeoniae Alba
- From:
Journal of International Pharmaceutical Research
2019;46(6):472-478
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the HPLC fingerprint of the chemical components of the decoction of Radix Paeoniae Alba(RPA)and determine the content of paeoniflorin, so as to provide a scientific method for the quality char- acterization and control of RPA and establish a correlation between the quality of standard granules in classical prescrip- tion and the quality of RPA. Methods: Thermo Syncronis C18(250 mm×4.6 mm, 5 μm)chromatographic column was used, the mobile phase was constituted by acetonitrile(A)and 0.05 % phosphate aqueous solution(B), the detection wavelength was 230 nm, the column temperature was 30℃, the flow rate was 1.0 ml/min, and the injection volume was 20 μl;the gradient eluting procedure was 0-15 min, 5% A-15% A;15-45 min, 15% A-25% A;45-65 min, 25% A-50% A. Results: The HPLC fingerprint of RPA was established, 11 common peaks were identified by HPLC-Q-TOFMS/ MS, and the similarity of 10 batches were evaluated. The content determination of paeoniflorin in 10 batches of RPA decoction-lyophilized powders was also completed. Conclusion: This study provides a scientific and reliable method for the quality characterization and control of RPA as well as a reference for the quality characterization and control of the standard decoction and granules.
