Analysis of the chemical constituents of volatile oil and the monosaccharide compositions of polysaccharide from Semen Ocimi Basilici
10.13220/j.cnki.jipr.2019.07.009
- Author:
Lian SUN
1
Author Information
1. Department of Chemistry of Pharmacy College of Xinjiang Medical University
- Publication Type:Journal Article
- Keywords:
Gas chromatography- mass spectrometry(GC- MS);
Polysaccharide;
Semen Ocimi Basilici;
Thin layer chromatography(TLC);
Volatile oil
- From:
Journal of International Pharmaceutical Research
2019;46(7):538-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To extract the volatile oil in Semen Ocimi Basilici(SOB),determine the chemical con- stituents of the volatile oil extract the polysaccharide from the SOB residue after extracting the volatile oil,and analyze the monosaccharide compositions of the polysaccharide. Methods: The SOB volatile oil was extracted by the simultane- ous distillation and extraction(SDE)method and by the conventional method of steam distillation,respectively,and the chemical constituents of the oils were analyzed and identified by the gas chromatography- mass spectrometry(GC- MS). Polysaccharides in the SOB residues were obtained by the water extraction,followed by the alcohol precipitation,and the monosaccharide compositions of the polysaccharide were analyzed by the thin layer chromatography (TLC). Results In total 51 compounds were identified in the SOB volatile oil extracted by the SDE method,accounting for 91.76% of the total oil amount,involving the alkanes,alkenes,alkynes,alcohols,esters,aldehydes,acids,ketones, ethers,acyl chloride,heterocycles,amide and other substances. In total 43 compounds were identified in the SOB vola- tile oil extracted by the conventional steam distillation,accounting for 88.20% of the total oil amount,which was related to the alkanes,alkenes,alkynes,alcohols,esters,aldehydes,acids,ethers,ketones,heterocycles,amides and other substances. The monosaccharide composition analysis showed that the SOB polysaccharide was composed of 5 kinds of monosaccharides. Conclusion: Among the identified compounds,only 9 compounds existed in the SOB volatile oils extracted by two different Methods:,indicating that the combined use of the two different extraction Methods: may be more powerful for comprehensive analysis of the chemical constituents of SOB volatile oil.
