Simultaneous determination of ten components in Shalian Hewei capsules by UPLC-MS/MS
10.13220/j.cnki.jipr.2020.01.011
- VernacularTitle: UPLC-MS/MS法同时测定砂连和胃胶囊中10种化合物含量
- Author:
Yi-Jia CAO
1
Author Information
1. School of Chinese Materia, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Content determination;
Shalian Hewei capsules;
UPLC-MS/MS
- From:
Journal of International Pharmaceutical Research
2020;47(1):72-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/ MS)method for the simultaneous determination of hesperidin, chlorogenic acid, ferulic acid, palmatine hydrochloride, jatrorrhizine, berberine hydrochloride, coptisine, synephrine, alantolactone and isoalantolactone in Shalian Hewei capsules. Methods: An acqui ty UPLC BEH C 18(2.1 mm×50 mm, 1.7 μm)column was used. The gradient mobile phase consisted of acetonitrile and 0.1% formic ac id. Mass data acquisition was performed with multiple reaction monitoring(MRM). Results : Excellent linear calibration curves were obtained for the ten components in the relevant concentration ranges(r=0.9991-0.9997). The precisions(RSD)for the ten compo nents were between 0.3% and 1.8%. The mean recoveries of the ten components were in the range of 91.5%-101.9%, with the RSD values of 1.1-5.1%. The contents of the ten components in 3 batches of samples were 5804.0-5825.6, 127.8-129.0, 26.6-27.0, 841.8-846.4, 439.6-443.2, 2797.2-2859.8, 776.8-800.2, 164.0-164.4, 1945.6-1952.8, and 2561.2-2578.0 μg/g, respectively. Conclusion: The compounds determined by this method are representative components in the compound preparation of Shalian He wei capsules, which could be used for quality and production process control of Shalian Hewei capsules.
