Preparation and in vitro evaluation of vitamin K1-loaded erythrocyte drug delivery system
10.13220/j.cnki.jipr.2020.06.007
- VernacularTitle: 红细胞载维生素K1给药系统的制备及体外评价
- Author:
Chao-Yang ZHOU
1
Author Information
1. School of Pharmaceutical Science, Hubei University of Science and Technology
- Publication Type:Journal Article
- Keywords:
Carrier;
Drug loading;
Encapsulation efficiency;
In vitro evaluation;
Red blood cells;
Vitamin K1
- From:
Journal of International Pharmaceutical Research
2020;47(6):444-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a vitamin K1(VK1)-delivery system with red blood cells as carrier. Methods: The VK1 self-emulsifying nano-emulsion, micelle and plasma solution were prepared to investigate the VK1 loading on red blood cells. The VK1- chitosan(CS)nanoparticles were prepared with different particle size by the 3 different preparation methods, and the effect of the particle size and charge property on the VK1-loading on red blood cells was investigated with the prepared VK1-CS nanoparticles. The encapsulation efficiency and drug loading were used as main indicators to investigate the appropriate drug loading method. Results: Due to the solubility of VK1 or the charge properties of nanoparticles, the drug loading and encapsulation efficiency of the red blood cell-encapsulated VK1 were quite low, and a large amount of drugs could not be loaded on the red blood cells. The ability of red blood cells to load VK1 was likely related to its Zeta potential. The VK1-CS nanoparticles prepared by the ion condensation method showed a good drug loading performance. Each milliliter of red blood cells could load 174.46 μg VK1 in the nanoparticles, and the loading rate was 85.11%. Conclusion: The VK1 loading by the red blood cells could be achieved by the electrostatic interaction between the positively charged chitosan nanoparticles and the negatively charged erythrocyte membrane.
