Simultaneous determination of pregabalin and preservative parabens in pregabalin oral solution by HPLC
10.13220/j.cnki.jipr.2020.07.015
- VernacularTitle: 高效液相色谱法同时测定普瑞巴林口服溶液中普瑞巴林及羟苯酯类抑菌剂含量
- Author:
Jing-Ru LI
1
Author Information
1. School of Pharmacy, Xuzhou Medical University
- Publication Type:Journal Article
- Keywords:
HPLC;
Methylparaben;
Pregabalin;
Propylparaben
- From:
Journal of International Pharmaceutical Research
2020;47(7):579-582
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC method for simultaneous determination of pregabalin, methylparaben and propylparaben in pregabalin oral solution. Methods: The analytical column was the CPACELL PAK C18 column(250 mm×4.6 mm, 5 μm). The mobile phase A was 25 mmol/L potassium dihydrogen phosphate solution(previously adjusted to pH 6.7 with concentrated ammonia)and the mobile phase B was methanol. Gradient elution was carried out at a flow rate of 1.0 ml/min. The detection wavelength was set at 210 nm for all analytes. The column temperature was maintained at 30℃, and the injection volume was 20 μl. Results: The linear ranges for pregabalin, methylparaben, propylparaben were 1000.45-4001.78, 65.21-260.84, and 8.17-32.68 μg/ml(all r≥0.9995), respectively. Their average recoveries were 100.17%(RSD=1.06%), 100.30%(RSD=0.56%), and 99.92%(RSD=0.28%)(n=9), respectively. Conclusion: The established HPLC method is simply operable, efficient, accurate and reproducible, which could be used for the quantitative determination of pregabalin and paraben preservatives in pregabalin oral solution.
